PROTEIN AND GLUCOSE VARIATIONS IN ENDOCRINE FLUIDS IN RELATION TO TESTICULAR OUTPUT IN POST PUBERTAL MALE GOATS IN THE TROPICS ⃰
DOI:
https://doi.org/10.33003/jaat.2025.1101.04Keywords:
west african dwarf, sokoto red, brain regions, testes, genotype, bucks.Abstract
The biochemical characteristics of cells and tissues are crucial for comprehending metabolic processes in animal organisms. This study evaluated sperm reserves and cerebral fluids in twelve bucks; six each from the West African Dwarf (WAD) and Sokoto Red (SR) breeds, weighing 7.5±0.61kg and 11.00±1.22kg, respectively. At 8weeks of observatory feeding, their reproductive tracts and brains were excised, homogenization, and biochemical assay conducted via standard procedures. Data was subjected to t-test analysis. There were no variation (p<0.05) in paired testis weight, daily sperm production, and sperm production efficiency between both breeds. Paired testis weight ranged from 6.83±0.51g to 7.53±0.45g, daily sperm production from 0.48±0.05 x 107 to 0.48±0.02 x 107 spermatozoa, and sperm production efficiency from 0.21±0.04 x 107 to 0.18±0.03 x 107 spermatozoa in post-pubertal WAD and SR bucks respectively. However, significant differences (p<0.05) were evident in epididymal regions between the two breeds, particularly in caput, corpus, and cauda reserves. Protein concentration in cerebral fluids varied significantly (p<0.05) across cerebral cortex, medulla oblongata, amygdala, pons varolli, midbrain, and hippocampus regions for both breeds. Total glucose exhibited similarities (p>0.05) within most brain regions, except in the hypothalamus. Interestingly, the hypothalamus region demonstrated analogous protein and glucose values in both breeds, implying a notable correlation (p<0.05) between in-situ hormonal activity in the brain and reproductive tract. In conclusion, this study evaluated the biochemical assay in reproductive tract and brain regions of WAD and SR bucks, emphasizing the significance of understanding these variances in the context of reproductive physiology.
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