• P.C EKE



Biological Treatment, Toxicity, Soil Leachate, Lentinus Squarrosulus, Mycofiltration, Automobile


This study was undertaken to investigate the mycofiltration of soil leachate from automobile repairs and servicing workshop Ikoku, Port Harcourt using Lentinus squarrosulus. To ascertain the contamination levels of the soil, sterilized sawdust was bagged, inoculated with mushroom spawn and allowed for 2 weeks to colonize the substrate. Then water extracted from the soil leachate was dispensed into the hole borne in the middle of the substrate then allowed to filter through into a sterile container which was subjected for physicochemical and microbiological analysis within 24 hours. Statistical analysis of data obtained after a 24-48 hours mycofiltration treatment, revealed a significant (P<0.05) reduction in the concentration of pH, Colour, Turbidity, Electrical conductivity, Total Dissolved Solid, Total Suspended Solid, Nitrite, Nitrate, Phosphate and Chemical Oxygen Demand. Electrical conductivity (µs cm-1) was reduced from 751 to 297, total dissolved solid (mg L-1) from 3.40 to 1.42, total suspended solid (mg L-1) from 2.81 to 1.07, nitrate (mg L-1) from 0.187 to 0.127, phosphate (mg L-1) from 1.04 to 0.72, turbidity level from 1028 to 401, biological oxygen demand (mg L-1) from 4.16 to 0, dissolved oxygen (mg L-1) from 401 to 0 and chemical oxygen demand (mg L-1) from 16.25 to 13.87. L. squarrosulus also exhibited a remarkable reduction in total heterotrophic count and total coliform count, which made it a potential purifying agent. The findings from this study showed that mycofiltration technique is a useful, efficient and affordable technology for removing pollutants from the soil in the automobile repairs and servicing workshop.


Abioye, O. P. (2011). Biological Remediation of Hydrocarbon and Heavy Metals Contaminated Soil. In (Ed.), Soil Contamination. Intech Open.

Adenipekun, C. O., Ayanleye, O. O., Oyetunji, O. J. (2013). Bioremediation of Soil Contaminated by Spent Diesel oil Using Pleurotus pulmonarius Fries (Quelet) and its Effects on the Growth of Corchorus olitorius (L). Journal of Applied Biosciences, 68: 5366-5373.

Adeniyi, A. A. and Afolabi, J. A. (2002). Determination of Total Petroleum Hydrocarbons and Heavy Metals in Soils within the Vicinity of Facilities Handling Refined Petroleum Products in Lagos Metropolis. Environment International 28(1–2):79–82.

Adesuyi, A. A., Njoku, K. L. and Akinola, M. O. (2015). Assessment of Heavy Metals Pollution in Soils and Vegetation around Selected Industries in Lagos State, Nigeria. Journal of Geoscience and Environment Protection, 3:11-19.

Adesuyi, A. A., Nnodu, V. C., Njoku, K. L. and Jolaoso, A. (2016). Evaluation of Industrial Discharge Point Source Pollution in Ikeja Industrial Estate, Ikeja, Lagos, Nigeria. Unilag Journal of Medicine, Science and Technology, 4(2):29-46.

Adesuyi, A. A., Njoku, K. L., Akinola, M. O. and Jolaoso, A. O. (2018a). Biomonitoring of Heavy Metals Level in Wetland Plants of Lagos Lagoon, Nigeria. Journal of Applied Sciences and Environmental Management, 22(9): 1489-1498.

Akpaja, E. O, Olorunfemi, D. I, (2014). Mycofiltration Effectiveness in Bioremediation of Contaminated Drinking Water Sources. Ife Journal of Science, 16(3): 35-43.

Azubuike, C. C., Chikere, C. B., Okpokwasili, G. C. (2016). Bioremediation Techniques Classification Based on Site of Application: Principles, Advantages, limitations and prospects. World Journal Microbioogyl. Biotechnology, 32:180. Chawaga, P. (2016). The Benefits of Biological Treatment for Drinking Water. Available: -of-biological-treatment-for-drinking-water00 01. Accessed 17 October 2019.

DataStream (2022). A Monitor's Guide to Water Quality Physical & Chemical Characteristics ( DSMZ (2007). Soil Extraction Medium. Available at: htt:// Retrieved on 13/11/2019.

Ekong, F. U., Michael, G. U. S. and Michael, U. S. (2012). Assessing the Effects of Mechanic Activities on Uyo Air Environment. Ethiopian Journal of Environmental Studies and Management, 5:1.

Fulekar, M. H. and Pandey, B. (2012). Bioremediation Technology: A New Horizon for Environmental.

Green, J. (2019). How Do Phosphates Affect Water Quality? Available at: Retrieved on 13/11/2019.

Igbojionu, V. O., Okwulehie, I. C. and Okwujiako, I. A. (2013). Mineral Composition of Engine Oil Polluted Soil After Degradation of the Oil by The White Rot Fungus Pleurotus florida (Mont.) Singer An Edible Fungus. Journal of Natural Product and Plant Resource, 3: 7-12.

Ikechi-Nwogu, C. G., Emmanuel, O. A. and Onyechere, V. U. (2020). Purification of Untreated Drinking Water using Lentinus squarrosulus. Mycopath, 18(2): 85-88.

Maduka, H. C. C. (2004). Water Pollution and Man’s Health. In Environmental Degradation, Reclamation, Conservation and Pollution Control for Rural Women and the Youths. Proceedings of the National Training Workshop organized by The Environment and Development Action Plan for Women (EDAPW) and the Nigerian National Commission for UNESCO (NATCOM), UNAD, pp.198.

National Environmental (Surface and Ground Water Quality Control) Regulation (NESGQCR) 2011. Physicochemical Ambient Water Quality Criteria. Available at: national environmental (surface and groundwater quality control) regulations,2010 ( accessed: 28/12/2022.

Njoku, K. L., Ogwara, C. A., Adesuyi, A. A. and Akinola, M. O. (2018). Vermiremediation of Organophosphate Pesticide (Dichlorvos) Contaminated Soil by Eudrilus euginae and Lumbricus terrestris. Environment Asia, 11(3):133-147.

Nwachuckwu M. A., Feng, H. and Alinnor, J. (2010). Assessment of Heavy Metal Pollution in Soil and their Implications within and Around Mechanic Villages. International Journal of Environmental Science & Technology, 7:347-358.

Ogbo, E M.and Okhuoya, J. A. (2008). Biodegradation of Aliphatic, Aromatic, Resinic and Asphaltic Fractions of Crude Oil Contaminated Soils by Pleurotus tuber-regium Fr. Singer- A White Rot Fungus. African Journal for Biotechnology, 7: 4291-4297.

Ogbo, E. M., Tabuanu, A. and Ubebe, R. (2010). Phytotoxicity Assay of Diesel Fuel Spiked Substrate Remediated with Pleurotus tuber-regium using Zea mays. International Journal of Applied Research in Natural Products, 3: 12-16.

Rhoda, C. J. (2014). Mycoremediation (Bioremediation with Fungi) - Growing Mushrooms to Clean the Earth Bioremediation with Fungi. Chem. Speciat. Bioavailab., 26: 196-198.

Robles-Hernandez, L., Gonzàlez-Franco, A. C., Crawford, D. L., Chun, W. W. C., (2008). Review of Environmental Organo-pollutant Degradation by White-Rot Basidiomycetes Mushrooms. Tecnociencia Chihuahua, 2(1): 3239.

Šašek, V. and Cajthaml, T. (2005). Mycoremediation: Current State and Perspectives. International Journal of Medicinal Mushrooms, 7(3): 360-361.

Sharma, S. and Bhattacharya, A. (2017). Drinking Water Contamination and Treatment Techniques. Applied Water Science, 7: 1043-1067.

Smith, M., Cross, K. Paden, M. and Laben, P. (2016). Spring - managing groundwater sustainably (PDF). IUCN. ISBN 978-2-8317-1789-0 09/08/2019.

Stamets P, 2005. Mycelium Running: How Mushrooms Can Help Save the World. Ten speed Press, Berkeley, Toronto. pp. 54-64.

Wandle Trust, (2014). Mycofilteration. Available at: Retrieved on: 4/10/2019.