ANTIOXIDANT EFFECT OF LYCOPENE ON IN VITRO MATURATION AND FERTILIZATION OF BOVINE OOCYTES
DOI:
https://doi.org/10.33003/jaat.2024.1004.08.386Keywords:
Antioxidant,, Lycopene,, maturation and, oocytesAbstract
In vitro embryo production (IVEP) is a vital assisted-reproductive technology with significant applications in producing genetically superior animals through in vitro maturation (IVM), fertilisation (IVF) and culture of zygote. This study evaluated the antioxidant potential of lycopene during in vitro maturation (IVM) and fertilisation (IVF) of bovine oocytes. Fifty ovaries from Belgian Blue cows were collected from a slaughterhouse in Moeskroen, Belgium, and transported to the laboratory. Cumulus-oocyte complexes were aspirated from follicles (4–8 mm diameter) and incubated in modified bicarbonate-buffered TCM-199 with supplements (50 µg/mL gentamycin and 20 ng/mL epidermal growth factor) at 38.5°C in 5% CO₂ for 22 hours. Four experimental groups were established: control (C), lycopene (supplemented with 0.2 µM lycopene), menadione (supplemented with 5 µM menadione), and lycopene plus menadione (L+M), each with 40 oocytes. Following maturation, oocytes were fertilized in media containing BSA and heparin with diluted sperm, co-incubated at 38.5°C in 5% CO₂ for 21 hours. Maturation and fertilisation were assessed under a fluorescent microscope after staining with Hoechst solution. The results showed that oocytes in the lycopene group (L) achieved significantly higher maturation (86.66 ± 5.09%) and fertilisation rates (84.99 ± 5.65%) compared to the other groups (p < 0.05). These findings suggest that lycopene supplementation enhances oocyte maturation and fertilisation outcomes. The study recommends incorporating lycopene into maturation media to improve IVEP efficiency in bovine reproductive technologies.
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