HAEMATOLOGICAL PARAMETERS AND ORGAN INDICES OF THE NILE TILAPIA (Oreochromis niloticus) FED WITH ROSELLE (Hibiscus Sabdariffa) SEED MEAL AT VARIOUS INCLUSION LEVELS
DOI:
https://doi.org/10.33003/jaat.2023.0904.17Keywords:
Packed cell volume,, haematological properties,, Oreochromis niloticus,, Hibiscus sabdariffa,, Inclusion levelsAbstract
Oreochromis niloticus, weighing an average of 0.60g, were reared in a plastic aquarium measuring 60cm x 50cm x 30cm with a water holding capacity of 120 litres for a period of 28 weeks. To examine the impact of different inclusion levels (0%, 25%, 50%, 75%, and 100%) of (Roselle) Hibiscus sabdariffa seed meal on the haematological properties of O. niloticus, five experimental diets were used in this study. At the end of the feeding trial, fish from each treatment group were randomly selected for haematological analysis. The blood of each fish was collected using a syringe and needle and stored in ethylenediamine tetra acetic acid (EDTA) bottles. This was used to determine various parameters: packed cell volume (PCV), red blood cell (RBC) count, white blood cell (WBC) count, haemoglobin (Hb) level, mean corpuscular haemoglobin (MCH), mean corpuscular haemoglobin concentration (MCHC), and mean corpuscular volume (MCV). The obtained results indicated that the fish fed with a 50% inclusion level of Roselle had the highest packed cell volume of 40.00%. The control group with 0% inclusion level 38.50%, followed by levels of 25%, 75%and 100 % (37.50%; 34.00%; 32.00%) respectively. There were significant differences (P < 0.05%) observed in all the PCV values. The RBC values exhibited a similar pattern with readings of 4.70 (50%), 4.35 (0%), 4.19 (25%), 3.37 (75%), and 3.10 (100%). The Hb values also followed a consistent trend, measuring at 7.70, 7.15, 8.20, 7.43, and 7.20 for inclusion levels of 0%, 25%, 50%, 75%, and 100% respectively. However, it is worth noting that lymphocytes and white blood cell counts were lower at the inclusion level of 50% (81.00) when compared to the levels of 83.50 (0%),84.45 (25%), and 89.70 (100%). Based on these findings, it can be concluded that processed Hibiscus sabdariffa seed meal can effectively replace fish meal in practical diet for O.niloticus at 50% inclusion level without any detrimental effects on the fish's haematological parameters.
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