ANTAGONISTIC POTENTIAL OF Trichoderma harzianum AGAINST F. oxysporum f. sp. lycopersici ISOLATES CAUSING FUSARIUM WILT DISEASE OF TOMATO (Solanum lycopersicum L.)
DOI:
https://doi.org/10.33003/jaat.2023.0901.19Keywords:
Antagonistic, Fusarim oxysporum, Inhibition, Isolates, T. harzianumAbstract
Antagonistic effect of T. harzianum was carried out on F. oxysporum f. sp. lycopersici. isolates of tomato variety (UC 82B) in a screen house located at the Teaching and Research Farm of Federal University of Agriculture, Makurdi during 2015 cropping season to determine the ability of the antagonistic in controlling Fusarium wilt diseases of tomato. The F. oxysporum isolates tested were coded as: FoAs1, FoAs2, FoAg, FoNb, FoSb, FoAm, FoAk, FoOr, FoAd and FoUAM together with an uninoculated control. The experiment was a 2 x 11 factorial laid out in Completely Randomized Design (CRD) and replicated three times. T. harzianum was introduced at three different times (Two days before, same time and two days after the inoculation of F. oxysporum). In vitro tests results revealed antagonistic effects of T. harzianum on F. oxysporum isolates. Growth inhibition was significantly higher (P≤0.05) when T. harzianum was introduced two days before inoculation of F. oxysporum. Interaction shows that T. harzianum introduced two days before inoculation of F. oxysporum gave better inhibition of all the Fusarium isolates tested except isolates FoAd and FoAg compared with when the antagonist was introduced at the same time and when it was introduced two days after inoculation of F. oxysporum. Also, T. harzianum (P≤0.05) totally inhibited the growth of isolates FoAg, FoAs1, FoNb, FoOr and FoUAM but not those of FoAd, FoAk, FoAm, FoAs2 and FoSb. It is therefore recommended that T. harzianum be used in the management of fusarium wilt disease of tomato
References
Agrios G. N. (2005). Plant Pathology 5th Edition Elsevier, London. Pp 948
Akrami M and Yousefi Z. (2015). Biological Control of Fusarium wilt of Tomato (Solanum lycopersicum) by Trichoderma spp. as Antagonist Fungi. Biological Forum – An International Journal, 7(1):887-892.
Anita, A. and Rabeeth, M. (2009). Control of Fusarium wilt of tomato by bioformulation of Streptomyces griseus in green house production. African Journal of Basic and Applied Sciences, 1 (1):9-14.
Arya, A. (2010) Recent advances in the management of plant pathogens: Botanicals in the fungal pest management. Commonwealth Agricultural Bureaux International, Aydi Ben
Abdallah, R., Jabnoun Khiareddine, H., Nefzi, A., Mokni Tlili, S. and Daami Remadi, M. (2016). Biocontrol of Fusarium wilt and growth promotion of tomato plants using endophytic bacteria isolated from Solanum elaeagnifolium stems. J. Phytopath., 164: 811-824
Cotxarrera L, Trillas-Gay M. I., Steinberg, C. and Alabouvette, B. T. (2002). Use of sewage sludge compost and Trichoderma asperellum Isolates to Suppress Fusarium wilt of Tomato. Soil Biology and Biochemistry 34:467-478.
Dissanayake, M. L. M. C. (2014). Inhibitory Effect of Selected Medicinal Plant Extracts on Phytopathogenic Fungus Fusarium oxysporum. Annual Research & Review in Biology, 4:133-142.
Egel, D. and Martyn, R. (2013). Fusarium wilts of water melon and other cucurbits. Plant Health Instructor, 10:1094.
Ekefan, E. J., Jama, A. and Gowen, S. R. (2009). Potential of Trichoderma harzianum isolates in biocontrol of Colletotrichum capsici causing anthracnose of Pepper (Capsicum spp.) in Nigeria. Journal of Applied Bioscience, 20: 1138 – 1145.
FAO. (2019). FAOSTAT statistical database. [Rome] : FAO. http://www.fao.org/faostat/en/#data/QC/visualize .Retrieved on 15/05/2021.
FAO (2020). FAOSTAT statistical database. [Rome]: FAO. http://www.fao.org/faostat/en/#data/QC/visualize .Retrieved on 12/05/2021.
Ghazalibiglar, H., Kandula, D. R. W. and Hampton, J. G. (2016). Biological control of fusarium wilt of tomato by Trichoderma isolates. New Zealand Plant Prot., 69: 57-63
Gwa, V. I. and Abdulkadir, K. H.. (2017). Biological Control Using Trichoderma harzianum against Penicillium purpurogenum, Causal Agent of White Yam Tuber (Dioscorea rotundata Poir) Rot J. Biores Commun 1:2 1-6.
Gwa, V. I. and Nwankiti, A. O. (2017). In-vitro Antagonistic Potential of Trichoderma harzianum for biological control of Fusarium moniliforme isolated from Dioscorea rotundata. Virology and Mycology, 6:166.
Gwa, V. I., Nwankiti, A. O. and Ekefan, E. J. (2019). In vitro study of antagonistic capability of Trichoderma harzianum against Aspergillus niger isolated from rotten white yam (Dioscorea rotundata) Tubers. Journal Advances in Biology and Biotechnology, 21(1):1-10
Gwa, V. I. and Ekefan, E. J. (2021). Biological control of postharvest fungal rot of white yam (dioscorea rotundata poir) tubers in storage with Trichoderma harzianum. Nigerian Journal of Mycology. 13:64-76
Gwa, V. I. Muhammad Akram, and Naveed Munir (2021). Effect of selected medicinal plant extracts and synthetic anti-fungal agent on post-harvest rot fungi of Hembankwase white yam (Dioscorea rotundata Poir) tuber cultivar. Nigerian Journal of Mycology, 13:1-11
Hermosa, R., Viterbo, A., Chet, I. and Monte, E. (2012). Plant-beneficial effects of Trichoderma and of its genes. Microbiol., 158: 17-25.
Houssien, A. A., Ahmed, S. M. and Ismail, A. A. (2010). Activation of Tomato Plant Defense Response Against Fusarium Wilt Disease Using Trichoderma Harzianum and Salicylic Acid under Greenhouse Conditions. Research Journal of Agriculture and Biological Sciences, 6(3):328-338.
Kutama, A. S., Aliyu, B. S. and Mohammed, I. (2007). Fungal pathogens associated with tomato wicker baskets. Science World Journal, 2:38-39.
Naika, S., Juede, J., Goffau, M., Hilmi, M. and Dam, V. (2005). Cultivation of Tomato Production, processing and marketing. Agromisa/CTA pp 252
Mamkaa, D. P. and Gwa, V. I. (2018). Effect of Moringa oleifera and Vernonia amygdalina Leaf Extracts against Aspergillus flavus and Botryodiplodia theobromae Causing Rot of Cowpea (Vigna unguiculata (L.) Walp) Seeds. Applied Science Research and Review, 5 (1,2):1-7 DOI: 10.21767/2394-9988.100067
Midhun, B. and Sobita, S. (2017). Efficacy of Trichoderma spp. against Fusarium oxysporum f. sp. lycopersici. (FOL) infecting pre-and post-seedling of tomato. Journal of Pharmacognosy and Phytochemistry 6(4): 616-619
Nwankiti, A. O. and Gwa, V. I. (2018). Evaluation of antagonistic effect of Trichoderma harzianum aginst Fusarium oxysporum causal agent of white yam (Dioscorea rotundata Poir) Tuber rot. Trend in Technical and scientific Research, 1(1):5555
Okigbo, R. N. (2005). Biological control of postharvest fungal rot of yam (Dioscorea spp.) with Bacillus subtilis. Mycopathologia, 159:307-314.
Pavlou, G. C. and Vakalounakis, D. J. (2005). Biological control of root and stem rot of green- house cucumber, caused by Fusarium oxysporum f. sp. radicis cucumerinum, by lettuce soil amendment. Crop Protection, 24:135-40.
Ritchie, B. (1991). Practical techniques in plant pathology. CAB International Wallingford.
Rojo, F. G., Reynoso, M. M., Sofia, M. F. and Torres, A. M. (2007). Biological control by Trichoderma species of Fusarium solani causing peanut brown root rot under field conditions. Crop protection, 26:549-555.
Sani, S. and Gwa, V. I. (2018).. Fungicidal Effect of Azadiracta Indica and Zingiber Officinale Extracts in the Control of Fusarium Oxysporum and Rhizoctonia Solani on Tomato (Solanum Lycopersicum) Fruits. Innovative Techniques in Agriculture, 2(4):439-448.
Siameto, E. N., Okoth, S., Amugune, N. O. and Chege, N. C. (2011). Molecular characterization and identification of biocontrol isolates of Trichoderma harzianum from Embu District, Kenya. Tropical Subtrop Agroecosystem, 13:81-90.
Suprapta, D. N. (2012) Potentials of microbial antagonist as biological control agents against plant fungal pathogens. J ISSAAS 18:1-8.
Downloads
Published
Issue
Section
License
Copyright (c) 2023 FUDMA Journal of Agriculture and Agricultural Technology
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
